FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44897568
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA473370.10543332770273882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG98000.014.07369938
AGCTTAG109150.012.777842545
GGCATAG111000.012.36297835
AGGGATC138400.012.0225662
CATAGGG117450.011.66470437
GTCACTC165300.011.1186218
GCATAGG127250.010.89005336
GGTCACT174600.010.6809897
GAGCTTA133850.010.48718144
TGGCATA134550.010.35004134
ACTCTCC190100.010.03607511
GGGAGGT205100.09.8692223
AGGTCAC198500.09.8029516
TCTATAG373350.09.5406773
GGAGGTC209500.09.467944
TTCTATA391400.09.3825742
CAGGGAT205050.09.291551
TAGGGGA200100.09.27698539
GGGATCA185050.09.1377593
GTTCTAT108900.09.0578221
Produced by FastQC (version 0.11.5)