FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33483645
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3286770.9816046012911677No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA526040.15710356503899142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG577350.028.8627783
AGACCGC577000.028.8486144
GACCGCG576000.028.8281155
ACCGCGT581000.028.6094556
GAACGAT574250.028.52632945
TCAGACC596900.028.3622362
CTCAGAC624550.027.802951
CCGCGTT604950.027.4875557
CGCGTTC609650.027.2569058
GCGTTCT645950.025.7704479
AGAACGA652550.025.19200744
CGTTCTC697850.024.15039410
AATACGG685350.024.06242632
ATACGGA684850.024.04692633
TACGGAG684950.023.93155934
CAATACG699200.023.66976531
AAGAACG695950.023.63394443
ACGGAGA705950.023.2451635
CCCAATA716100.023.24104929
CCCCAAT733100.022.70892328
Produced by FastQC (version 0.11.5)