FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33483645
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT511240.1526834966742719No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA421670.125933123469682No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG400840.1197121758996071No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG389650.11637024583195767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC204550.014.2216528
AGCTTAG154000.014.21774545
GGTCACT205600.014.127397
GGCATAG157600.013.74945135
ATAGGGG158950.013.61723538
ACTCTCC223200.013.07415711
TGGCATA169100.012.94747634
GGGAGGT237700.012.8921563
GAGCTTA173200.012.65136744
GAGGTCA242700.012.5523565
GGAGGTC243700.012.4273224
CATAGGG178350.012.2497837
CACTCTC239750.012.24666610
TCACTCT248400.012.082719
AGGTCAC251450.012.00760656
AGGGATC124450.011.8059022
ACCCGGC58700.011.7672945
GCATAGG187550.011.60160836
TAGGGGA261100.011.185060539
AAGCCGG175050.011.05541245
Produced by FastQC (version 0.11.5)