FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51999280
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1254650.24128218698412748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG36650.024.4366726
CGGTATA66250.021.25902620
TCGACGA43850.021.1902147
CGACGAG44750.020.060378
AGACCGC94600.017.4132774
GACCGCG96100.017.2819655
CGGCAGT393600.016.48558240
ACCGCGT103750.016.006686
GTTCGAC57800.015.963065
GACGGCA415500.015.61120638
CCGCGTT169600.015.5938891
TGACGGC419550.015.50342437
ATGACGG89050.015.36166616
GTCCGTT39450.015.0625791
CTGACGG439700.014.80824636
GTATAGA98300.014.78542722
ACGGCAG441250.014.75137139
ACGAGGA85500.014.68494110
AGTTGCA446650.014.58290644
ACGCTAC186450.014.528439524
Produced by FastQC (version 0.11.5)