FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42981821
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1505980.3503760345565629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC285450.026.515424
ACCGCGT287900.026.2350736
GACCGCG290350.025.9362075
CAGACCG300000.025.4095063
TCAGACC328900.023.8746592
CCGCGTT315600.023.8681957
CGCGTTC327850.022.9763668
CTCAGAC391950.021.279831
GAACGAT370350.020.42720845
GCGTTCT374900.020.1708229
AGAACGA408550.018.59757844
ATACGGA418450.018.0446733
AATACGG419350.018.02210232
TACGGAG417500.017.93483734
CAATACG432650.017.60340131
AAGAACG433450.017.5240343
CGTTCTC450800.017.21392310
ACGGAGA445450.016.89537235
TTCGACG16200.016.805176
CCCAATA471150.016.39899429
Produced by FastQC (version 0.11.5)