FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42981821
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA466470.10852727714816923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG102800.014.60213638
AGCTTAG104350.014.35102145
CATAGGG111450.013.46967837
GGCATAG111300.013.36818335
GCATAGG122250.012.28080736
GAGCTTA128350.011.807693544
TGGCATA133200.011.32299834
TAGGGGA180000.010.4508439
ACCGGTA30100.010.38363445
AGGGATC117100.010.3498232
GGGAGGT208600.010.164793
GTCACTC189950.010.0622098
GGTCACT192250.09.9298437
GCGTTCG23000.09.78149315
GGAGGTC212450.09.6101494
CGCGTTC25200.09.19465314
TACCGGT33750.09.19403844
GCATTCG45050.09.0340993
ATTTCTA255800.08.9220892
ATAGCTC52750.08.91540839
Produced by FastQC (version 0.11.5)