FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47811749
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1110400.23224417077902754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC212350.024.7637834
GACCGCG214650.024.5089195
CAGACCG218600.024.1689873
ACCGCGT219150.024.0361066
CCGCGTT252450.020.8030687
CGCGTTC261700.020.0923638
TCAGACC269300.019.978122
CTCAGAC322000.017.960791
GCGTTCT316350.016.755919
CGTACGC39200.014.809635
CCGTACG39700.014.679794
CCCGTAT32800.014.339061
GTCCGTT35900.014.1664891
CGTTCTC388400.013.88511310
CTCCGAT52850.013.8810031
TACGCCA46500.013.0650997
GAACGAT407700.012.71477645
CTCGGTT72700.012.5053311
ACTCCCC426900.012.49147325
CTCGTTT90700.012.4302161
Produced by FastQC (version 0.11.5)