FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44057427
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA485560.11021070295366998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA115150.013.34598745
GTTGTCG25350.011.53966926
TATATAC55500.011.1608247
ATAGGGG96050.011.05098138
CATGCAT81150.010.9914089
TAATCGG79400.010.9226714
GTAACGA76150.010.82181918
GGTAACG77350.010.71280817
ATCGGTT85150.010.5546736
AATCGGT83050.010.4968495
ATAATCG83700.010.2538553
GGCATAG99050.010.05624635
CGTAACG11450.09.82347742
TACGGTT42000.09.68982535
TCGGTTT96050.09.4272547
CATAGGG107300.09.26254737
GTGTCGA91350.09.13075427
TGTTACG46950.09.09719632
TGTCGAG97150.09.09421828
ATATATA79350.08.9984746
Produced by FastQC (version 0.11.5)