FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24537405
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT323450.1318191552855732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG91150.023.2801425
ACCGCGT93550.023.1396876
AGACCGC100200.021.851314
CAGACCG101300.021.7917423
CCGCGTT101550.021.4718787
CGCGTTC107600.020.2431768
TCAGACC132900.018.6082322
CTCAGAC164800.016.8763871
GCGTTCT129050.016.823899
CGTTCTC165300.014.60444110
GAACGAT145050.014.42542345
TTCGACG7450.014.1960576
CCCGTAT15800.013.5295931
AATACGG160000.013.45823232
TACGGAG154600.013.24362134
ATACGGA163600.013.21693833
CAATACG174800.012.80789531
TCGTTAC16200.012.77708344
CGTTACG10200.012.35297521
AGAACGA170500.012.3380444
Produced by FastQC (version 0.11.5)