FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45988448
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG52100.022.41075144
TCGGCGC54950.021.44750445
CCGTACG56700.020.9972924
CGTACGC58750.020.1874795
AGACGCT163400.019.99116141
CCCCGCA158000.019.92075735
AAGCGGC158000.019.6806828
CCGCAGA171800.019.45908737
CTCGGTT110100.019.3789441
CCCCCGC169800.018.81521234
GCAGACG172900.018.81465739
CGCCCCG63100.018.792523
ATACGGA63400.018.7711478
TACGGAG62550.018.7024339
TACGCCA64150.018.6223347
CGCGCCC63600.018.60899721
CGGGGCG64200.018.29361733
CGCGGGG63950.018.2247631
CTCGCGT10800.018.1302831
AGCGGCC172900.018.0753629
Produced by FastQC (version 0.11.5)