FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45988448
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCG21000.011.67883920
ACCCGGC62550.011.2950395
ATAGGGG52300.010.8418938
GCGTTCG24700.010.47435415
CATTCGG56550.09.9074214
CTTAGAT78450.09.780841
TGCCCGT97050.09.52712344
TCACCCG93450.09.4384593
TCGCGCT47600.09.40842845
ATAGCTC68250.09.3958139
ACGGACA76200.09.33025318
CGCGTTC29500.09.15143914
TACGGGT25600.09.05174116
CATAGGG61450.09.04445137
TAGGCTA67400.08.9447410
CCGCGTT31600.08.89892413
CGTAACG15700.08.74129842
CCTAGGC70500.08.6791728
GCATTCG59650.08.6758443
CACCCGG88250.08.4393154
Produced by FastQC (version 0.11.5)