FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40159141
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACG24000.015.65512310
AGACCGC30900.014.8532994
AACGCTA123000.014.76181223
ACGCTAC123950.014.73893624
TTCGACG13000.014.1912856
CGCTACC125600.014.16896325
CCGTACG52800.013.9336574
CAGACCG35000.013.6918723
CGTACGC56150.013.3427625
AAAACGC138050.013.12042921
GACCGCG35600.013.08192355
CGGAAAT42750.012.8988351
CGGCCTA30900.012.523378
CCTACGT33300.012.499111
AAACGCT146600.012.33979422
CGTGGTT146350.012.3153541
GAAAACG148750.012.2976520
CGCGTTT11350.012.2946551
CCGGTAT37750.012.2820471
CCGGCAT45650.012.1287171
Produced by FastQC (version 0.11.5)