FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24324651
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG279600.1149451229536654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24750.029.3597986
TCGACGA28150.025.8141027
CGACGAG29150.024.6198278
GTTCGAC30900.023.881115
CGGTATA38450.023.815120
AGACCGC63800.021.7576794
GACCGCG64100.021.515015
CAGACCG69050.020.3654443
ACCGCGT68800.020.1100166
AGTTCGA39300.019.2351154
AACGCTA119850.018.66153323
CAGTTCG42350.018.6479573
ACGCTAC120650.018.59397124
CGCTACC123700.018.0445625
TCAGACC86900.017.7367212
AAAACGC132200.017.15506421
ACGAGGA52900.016.9258510
CGAGGAT48750.016.56699811
ATGACGG50300.016.503516
GAAAACG135350.016.4895120
Produced by FastQC (version 0.11.5)