FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45185436
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG599950.13277508266159035No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA574410.1271228189543197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC119800.018.0715775
ATAGCTC125450.016.33755739
TCACCCG137650.016.039253
AGCTTAG134550.015.90190745
CGGACAC136950.015.59306713
ACGGACA139400.015.52764218
TAGCTCT136150.015.21893540
GGCATAG142300.015.14545235
CCGGACA142300.015.0860212
CACCCGG148500.014.7913554
CATAGGG149100.014.46986637
CGGACAG149750.014.24407619
CACGGAC154250.014.06196717
TGATAGC150700.013.89824537
GACACGG154250.013.84354415
GCATAGG157750.013.6763736
ACACGGA156100.013.65045216
TTGATAG155550.013.63839636
ATAGGGG163050.013.50803638
GTCACTC208850.013.50068958
Produced by FastQC (version 0.11.5)