FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33391293
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN656650.19665306162298057No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA539360.1615271382273217No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG530370.15883481960402074No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG462180.13841332828890454No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG364800.10925003712794232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG170750.016.23222245
GGCATAG190800.014.519011535
ATAGGGG194350.014.48224238
GGGAGGT274050.014.0013623
AGGTCAC268050.013.9871496
GGAGGTC273500.013.8317124
GTCACTC264750.013.7104428
GAGGTCA287050.013.3742855
TGGCATA209650.013.35160634
GGTCACT277350.013.2907027
GAGCTTA210400.013.27939244
ACTCTCC282800.012.96195711
TCACTCT288850.012.8306749
GTTGTCG21300.012.45496726
CACTCTC295750.012.22702210
CATAGGG234750.011.98753537
GCATAGG243600.011.57654836
CACCCGG76900.011.508211
ACCCGGG73400.011.2593562
TGGGAGG269900.010.9798542
Produced by FastQC (version 0.11.5)