FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34978436
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG119400.011.55182445
ATAGGGG129550.010.76234738
GTCACTC173100.010.4483458
GGGAGGT180800.010.4155233
GGCATAG133500.010.24018935
AGGTCAC185650.010.166866
GAGCTTA135350.010.13947944
GGTCACT178050.010.1201087
TCAGACG54100.09.8135852
ACTCTCC191350.09.56357111
TGGCATA152650.09.13229334
GAGGTCA212300.08.9974685
CCGTACG7400.08.8275614
GGAGGTC218750.08.6703874
CATAGGG165850.08.66354937
TCACTCT216400.08.5970969
GCATAGG171400.08.33065136
TCTATAG131900.08.3168455
ACCGGTA19750.08.20275545
CTATAGT137300.08.169526
Produced by FastQC (version 0.11.5)