FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44514556
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26750.011.77560126
ATAGGGG84050.010.17312938
TACGGTT45600.09.32743635
GGCATAG88800.09.32610235
CGACCCG46200.09.204276
TCTATAG191600.09.0304663
TTACGGT49550.08.99266234
TGTCGTT36750.08.87805628
TTACTAG190950.08.71903514
TACTAGA195950.08.62288315
TTCTATA216150.08.6189132
CGTGGCG48350.08.5624341
CTATAGT201200.08.5214234
TGTTACG51600.08.4173432
GTCGTTC37900.08.37196929
TATAGTG213700.08.3074455
ATATAGC51550.08.205386
CATAGGG100450.08.19909137
GGGAGGT158300.08.115863
GGTAACG77350.08.11524717
Produced by FastQC (version 0.11.5)