FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43852001
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG733920.16736294428160758No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA648680.1479248347184887No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG443160.10105810222890399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33850.015.38960326
ATAGGGG120550.013.66430638
GGCATAG117500.013.21471535
AGCTTAG118000.013.15947545
CATAGGG131650.012.03375837
TATAGCT54450.011.9227747
TGTCGTT44250.011.67150828
AGGGATC156850.011.4523612
GCATAGG138150.011.41849236
TGGCATA139800.011.3461334
GTCACTC174300.011.22357758
GGTCACT175600.011.1677877
ATATAGC55850.011.0613396
TTGTCGT50700.010.85079727
GAGCTTA146950.010.75442844
GGGAGGT202200.010.6509993
ATTTCTA392800.010.594192
GCTAAGT52150.010.33197114
GTCGTTC49850.010.3149829
GGAGGTC206950.010.2107244
Produced by FastQC (version 0.11.5)