FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46279371
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA648210.14006456570034195No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG546170.1180158649952265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG117300.014.89471438
GGCATAG125150.013.65496235
AGCTTAG125800.013.64490345
CATAGGG135450.012.64984237
GCATAGG145650.011.84125736
GAGCTTA149950.011.79178744
GGGAGGT211400.011.390373
GGAGGTC209850.011.0874464
GTCACTC202050.010.9539518
TGGCATA163150.010.72209534
AGGGATC138250.010.3597982
ACTCTCC215100.010.29527311
GGTCACT216200.010.1956277
GAGGTCA242300.010.0206345
GTTGTCG27650.010.01045926
CACCGAA344500.09.91309145
AGGTCAC237900.09.8524166
TAGGGGA224100.09.81331139
GCACCGA361900.09.38624644
TCTATAG403550.08.7074783
Produced by FastQC (version 0.11.5)