FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20764097
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC28700.023.9853234
GACCGCG30750.022.3863035
ACCGCGT30900.022.2048266
CAGACCG31650.022.0340353
TACGCGA1358.61688E-716.663498
CCGCGTT50550.015.4106851
TCAGACC49900.014.7869742
CTCAGAC68150.014.2389641
TTCGACG6100.013.6451386
CCGGTAT15350.013.6408551
CTCGTTT33300.013.5899811
CGTACGC11500.013.1063875
CCGTACG11400.013.0240234
CGCGTTC59800.012.9032452
CTCCGAT18200.012.3707371
CTCGGTT26400.012.3660531
CGCGTTT5650.012.3532211
CCGGATT22050.012.1508131
CTCGTAT20750.011.8270221
AACGCTA49300.011.82484123
Produced by FastQC (version 0.11.5)