FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20764097
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG14650.019.49405326
ATAGGGG40800.015.33947538
ATATAGC26000.014.0027616
GGGAGGT66600.013.8569553
TGTCGTT20500.013.82212428
GGCATAG47050.013.487802535
GCTAAGT22200.013.46889714
CGTGGCG32700.013.39222051
AGCTTAG51900.012.44264645
CGACCCG37950.012.3175276
TCGTTCC24650.012.13521130
GTGGCGA35300.011.9528912
TATAGCT29650.011.9001147
GTCGTTC24550.011.81747229
TTGTCGT24500.011.74888627
CATAGGG55750.011.670073537
GGCGACC37700.011.49154954
GGAGGTC79300.011.267844
GCATAGG57050.011.20443436
CGTTCCC26300.011.20311131
Produced by FastQC (version 0.11.5)