FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51186666
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2216070.4329389220231691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC436200.025.3728034
CAGACCG446100.024.875293
GACCGCG443300.024.8598425
ACCGCGT445350.024.7807756
TCAGACC489900.023.3073962
CCGCGTT498250.022.1260157
CTCAGAC551800.021.6203581
CGCGTTC514900.021.4186928
GCGTTCT561050.019.7410359
CGTTCTC637950.017.78810510
GAACGAT629200.017.51699345
GTTCCTA91050.016.6788115
TTCGGCG66250.016.5754944
TCGGCGC67250.015.96083545
AGAACGA693950.015.918323544
CCTCTCA729750.015.44252119
AATACGG717050.015.36956232
ACTCCCC737550.015.29525125
ATACGGA721500.015.28416833
TACGGAG715700.015.26685534
Produced by FastQC (version 0.11.5)