FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51186666
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA832640.16266736341061946No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG678920.13263610487934493No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG538520.10520708654867265No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG512020.10002995702044747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG176450.015.36414445
ATAGGGG190500.014.58527338
GGCATAG190200.014.22976435
GGAGGTC282000.013.2028824
GGGAGGT287650.013.1713183
CATAGGG210300.013.05158137
GAGCTTA209350.012.98184944
TGGCATA211450.012.916759534
GTTGTCG24250.012.33908326
GCATAGG223750.012.28713836
ACTCTCC318100.011.30930311
TAGGGGA325800.010.98655839
AAGCCGG256650.010.51044145
CACTCTC352550.010.31932710
AGGGATC178050.010.2416512
TCTCCCC351650.010.22379513
GCTCTGT360600.010.15090622
TGGGAGG295250.09.9886842
GTCCAAG374700.09.96706727
GGTCACT362350.09.9182417
Produced by FastQC (version 0.11.5)