FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38592099
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA555070.14382995856224354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG21850.016.67352526
ATAGGGG103700.014.89457438
GGCATAG107550.014.18789335
AGCTTAG108000.014.14588145
CGCGTTC25200.013.02598614
GCGTTCG25100.012.98830915
CGTTCGC26150.012.81126616
TGTCGTT27850.012.75863428
TGGCATA122050.012.666334
GGGAGGT196300.012.5817343
CCGCGTT27100.012.52834213
ATATAGC34150.012.4365816
GCGTAGG59650.012.41500136
GTCGTTC30500.012.38911929
GAGCTTA125750.012.29652444
CATAGGG124850.012.28121437
GGAGGTC194550.012.2302744
GGCGTAG60800.012.14140335
CGTAGGG61850.012.085958537
TTTCTAT273150.011.6465591
Produced by FastQC (version 0.11.5)