FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20998743
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG532270.25347707717552426No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA456300.2172987211663098No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG381450.1816537304161492No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG336850.16041436385025523No Hit
CTACAATATAGCTGCTAAGTGCTGTGTTGTCGTTCCCCCTGCTTAAAATAA267500.127388577497234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252610.12029767686570572No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA218240.10393003047849102No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT212370.10113462505827134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC64050.022.850216
GTTGTCG61850.021.21532426
TATAGCT70900.020.7720037
GCTAAGT70100.019.6757414
TGTCGTT68050.019.38546828
TACAATA80050.018.864522
CAATATA80100.018.7176744
ATAGCTG78750.018.2469648
TCGTTCC73200.018.11449430
CGTTCCC73300.017.9149831
TTGTCGT74800.017.84423627
GTCGTTC75300.017.60918229
TAGCTGC81000.017.5763459
CTAAGTG79450.017.50179715
ACAATAT91700.017.0818963
AATATAG93100.017.0211895
TAAGTGC82650.017.0145916
GGAGGTC156200.016.8979594
GGGAGGT157750.016.630113
AACTCTA70850.015.8442545
Produced by FastQC (version 0.11.5)