FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40817509
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGC548090.13427815989456876No Hit
CTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGCA491550.12042626119100017No Hit
CTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTA485830.11902490178908273No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG480650.11775583855447916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC66050.024.5571164
ACCGCGT66800.024.414766
CGGTATA27000.022.83022520
TTCGACG16950.022.8270446
CCGTACG27000.022.6631374
CGTACGC28450.021.5859585
GACCGCG75250.021.5834885
CAGACCG76400.021.5247883
CCGCGTT76150.021.2986247
TATGCCG22400.021.29357145
TCGACGA20550.019.3752617
CGCGTTC87800.018.5750358
TACGCCA35450.017.9579337
GTTCGAC21200.017.9325395
CTCAACT198000.017.7979131
CGACGAG22450.016.8336818
TCAGACC109400.016.3274382
CTCCGTT55000.016.0488281
CTCGGTT67200.016.0169031
AACGACG14000.015.58582816
Produced by FastQC (version 0.11.5)