FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23356345
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG509310.21806065974791863No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA347220.14866195888098074No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC290930.12456144144128715No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGG277940.11899978357058863No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG270310.11573300531397357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT243900.017.7026713
AGGTCAC245300.016.9577626
GAGGTCA250400.016.9178495
GGAGGTC250450.016.7632374
GGTCACT248700.016.5363567
CTGGGAG185900.016.4853611
GTCACTC252700.016.1678338
TGGGAGG181000.015.3897212
CCGGGAG110350.015.2928941
CGTGGCG70350.015.2565051
ACTCTCC270400.015.11857511
TCACTCT279750.014.8137019
GTGGCGA72950.014.6198092
CACTCTC278950.014.52613210
AGGGATC63250.014.3361712
GTTGTCG11650.013.90598426
TGGCGAC78500.013.9010533
TAGGCTA41900.013.80008410
CGACCCG81100.013.4262176
GCGACCC82450.013.3428125
Produced by FastQC (version 0.11.5)