FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41182633
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1081190.2625354236092675No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG577710.1402800058947178No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA511830.12428297141661633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC176450.030.5032064
CAGACCG179150.030.131413
ACCGCGT181450.029.5474016
GACCGCG182050.029.4042265
TCAGACC223250.025.0663052
CTCAGAC277800.021.9897461
CGCGTAA11700.021.7387811
CCGCGTT248450.021.605237
CGCGTTC285200.018.797588
CCGTACG67850.017.941284
CGTACGC69000.017.5770385
GCGTTCT333150.016.1933569
TTCGGCG76100.015.67044744
TCCGTAC78100.015.6154423
TACGCCA81000.015.1925177
CTCGGTT74650.014.9552791
TCGGCGC83700.014.40885745
GTACGCC85400.014.3578986
CGTTCTC395750.013.893373510
CGCCGCG85000.013.87273728
Produced by FastQC (version 0.11.5)