FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51214067
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2347210.45831353327202073No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATATGGAGAGAAGAACGAT591660.11552685319836052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC517300.028.5525534
CAGACCG525100.028.0556853
GACCGCG536850.027.4336515
ACCGCGT538550.027.430116
TCAGACC583300.025.9508842
CTCAGAC622650.025.3738141
CCGCGTT597150.024.7173547
CGCGTTC617950.023.8744538
GCGTTCT650550.022.711689
CGTTCTC727450.020.85203710
GAACGAT757800.019.22336645
GTTCTCT837450.018.25011611
CCTCTCA838000.017.9211719
AGAACGA824900.017.7169544
ACTCCCC860200.017.3997625
TCACTCC883200.017.05354123
CCCAATA874950.016.96522529
AAGAACG867750.016.82392743
AATACGG692600.016.7852832
ATACGGA693800.016.74000233
Produced by FastQC (version 0.11.5)