FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33949766
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG96400.011.40795438
AGCTTAG97100.010.98432245
GGCATAG101050.010.61380235
GTCACTC131950.010.1043758
GGTCACT137250.09.5175187
CATAGGG115900.09.390552537
ACTCTCC146850.09.308454511
GAGCTTA120250.09.18700644
TGGCATA120000.09.16260734
GCATAGG120750.08.95741536
GGAGGTC156150.08.8566944
AGGTCAC156150.08.8415126
GGGAGGT160800.08.7969013
TAATCGG31200.08.5186864
AGGGATC90950.08.4100162
GTAGCGT76350.08.36995945
TCACTCT167250.08.30841359
CACTCTC162200.08.30271410
GAGGTCA173650.08.2754035
TCAGACG50300.08.2741682
Produced by FastQC (version 0.11.5)