FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49211889
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1971960.40070804841488605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG342100.029.221955
AGACCGC348350.028.8010034
ACCGCGT349500.028.6611716
CAGACCG358700.028.0954273
CCGCGTT379650.026.2477937
TCAGACC402800.025.8914492
CGCGTTC395400.025.236438
CTCAGAC470500.023.2248441
GCGTTCT454950.022.0073039
GAACGAT483350.020.9133545
TTCGACG20750.019.5182276
AGAACGA519400.019.51478644
AATACGG527550.019.22616232
ATACGGA528050.019.18665133
TACGGAG525650.019.15866534
AAGAACG537900.018.83102843
CAATACG540900.018.78491631
CGTTCTC551450.018.682510
TTCGGCG53850.018.55103944
ACGGAGA555950.018.19944435
Produced by FastQC (version 0.11.5)