FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44828835
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1088800.242879387786901No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA458800.1023448412165964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC225950.024.3764724
CAGACCG233200.023.7250733
GACCGCG231350.023.7102415
ACCGCGT237650.023.1384966
TCAGACC279650.020.7017422
TTCGACG25100.020.2584636
CTCAGAC340250.018.333571
CCGCGTT306900.017.895377
CGCGTTC328300.016.7151688
CGGTATA38200.016.3748720
TCGACGA35300.014.6596287
GCGTTCT379550.014.5944739
CGACGAG36050.014.2922328
CCGTACG74700.014.0960354
CGTACGC75200.013.9125555
GTTCGAC35950.013.7061985
TTCGGCG75700.013.67197644
CGTTCTC432450.013.24101410
TCGGCGC78950.013.11109445
CCCGTAT25550.012.9519491
Produced by FastQC (version 0.11.5)