FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44828835
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA760050.1695448922551746No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG693250.15464376890454548No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG605130.13498677804141018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG211950.016.29422645
ATAGGGG225400.015.30655938
GTCACTC333850.014.9786078
GGTCACT337050.014.6558717
GGCATAG234350.014.63858235
GGGAGGT356450.014.6025363
GAGCTTA240150.014.36212444
TGGCATA242800.014.17630534
ACTCTCC357450.013.99121811
GGAGGTC369500.013.9590194
AGGTCAC386300.013.235546
CATAGGG263750.013.23525937
GAGGTCA395800.013.1280465
TCACTCT387650.013.0390829
AGGGATC170050.012.7857512
CACTCTC396100.012.51732910
TAGGGGA404050.012.24090539
GCATAGG292100.011.94438236
TCTCCCC423650.011.81377413
GCTCTGT430750.011.71330622
Produced by FastQC (version 0.11.5)