FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39721077
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1961520.4938234680796797No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA740800.18650048184745846No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG582100.14654688240200536No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG445020.11203623708390385No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCC430230.10831277309021606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG28500.025.3243856
AGACCGC78300.022.8863144
CGGTATA44650.022.33444620
TCGACGA33700.021.4834847
CAGACCG86750.020.838473
TTCGGCG82950.019.95223444
CGACGAG34400.019.935288
GACCGCG90550.019.715665
TCGGCGC84550.019.62807845
CCGCGTT190150.019.3958241
ACCGCGT101800.017.5810186
CGGCAGT591650.016.84088140
CGCGTTC218550.016.7589932
TTCGCGC12800.016.5118837
TCAGACC118100.016.2967682
CTACACG53200.016.27361945
TGACGGC613900.016.23398437
AGTTGCA628100.015.99253444
GACGGCA623550.015.97523638
ATGACGG60000.015.73876916