FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25063639
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG360850.1439735068000301No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC288410.11507107966245445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG13950.021.4391776
CGGTATA22350.018.32011220
CGTACGC64650.017.6346765
CTCGGTT61150.017.1421661
TCGACGA17850.017.132927
GTCCGTT17750.016.981761
CCGTACG67950.016.7451524
CGCCGCG69350.016.29071228
TCCCGCG70900.016.1432818
GACCGCG37900.016.0789665
CGACGAG18650.016.036058
CGGGGCG71000.016.0005333
GTACGCC72700.015.8367566
AGACGCT133150.015.55636141
TACGCCA74550.015.4135927
TCCGTAC76350.015.3740943
TCGGCGC75450.015.17269945
ATTCGGC76700.014.9842543
CGCGGGG76100.014.89982131
CCCCGTT40750.014.8491251
Produced by FastQC (version 0.11.5)