FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34665354
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3349190.9661490836066466No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA581240.16767173357006537No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG422680.12193154006158424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC592000.028.0927034
CAGACCG596250.027.964893
ACCGCGT605250.027.5119826
TCAGACC616550.027.3689232
GACCGCG608650.027.311745
CCGCGTT645750.025.8526557
CTCAGAC683450.025.6593511
CGCGTTC666900.025.0155748
GAACGAT658650.024.83004445
GCGTTCT703350.023.760569
AGAACGA729500.022.5244
CGTTCTC777100.021.76907710
AATACGG770500.021.39932
ATACGGA772200.021.31634333
AAGAACG775500.021.19872943
TACGGAG772050.021.19514334
CAATACG785600.021.0767531
GTTCTCT815950.020.79324511
ACGGAGA795100.020.6286535
CCCAATA809800.020.59259429
Produced by FastQC (version 0.11.5)