FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34665354
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT698350.20145474354596235No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA563470.16254557792774885No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG538220.1552616482727971No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC406880.11737367516858475No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG377280.10883489030575023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG90750.013.21666545
ATAGGGG96400.012.32229238
GGCATAG100100.011.93402535
CATAGGG109200.010.9603337
TTTCTAT292600.010.8239721
GAGCTTA115300.010.6568144
TGGCATA112100.010.65644334
TTCTATA296900.010.5748682
GCATAGG119900.010.03850936
AGGGATC112600.010.0140622
AAGCCGG279050.09.74957345
ACTCTCC166700.09.71537111
GGGAGGT184550.09.5854233
ATAGCTC41250.09.49489739
TATCCTA48100.09.4515875
GGAGGTC181600.09.3938644
CTATAGT334600.09.2618744
ATTTCTA224650.09.2471152
TAGGGGA167650.09.19712239
TAGGGAC108700.09.17313542
Produced by FastQC (version 0.11.5)