FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116048
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGG50890.45598397201554053No Hit
GTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGAGG48710.4364507619743953No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG39860.35715309735781975No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG36930.3308997462474732No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG31920.2860092039052084No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCA30690.2749881725517182No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30370.2721209123621923No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30290.2714040973148108No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCA27650.24774920075122217No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCC27650.24774920075122217No Hit
GTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAG27090.24273149541955186No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGG25600.22938081516207187No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC25220.22597594368700988No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23830.21352128223875674No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23760.212894069072298No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23380.20948919759723594No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23250.20832437314524105No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTAACAGATCTCAGCTTCAGGGA23120.20715954869324615No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22970.20581552047940593No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC21850.19578010981606528No Hit
ATAATACACAGCCGTGTCTGCGGCGGTAACAGATCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTCCGGA21810.19542170229237452No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21690.1943464797213023No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG21200.18995598755609078No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21070.18879116310409588No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20850.18681992172379683No Hit
GTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACC20670.1852070878671885No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19990.17911415996444596No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19750.17696371482230155No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19410.17391725087093027No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19030.17051237939586827No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19020.17042277751494558No Hit
GTATTATTGTGTGACATATAGTTCGAATATCGCAGCTCCCCGGGACCACTACTGGGGCCAGGGAACCCTGGTCAC19010.17033317563402292No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18650.16710750792080628No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTTTATTGGTACCAGCAGCTCCCAGGAACGGCCCC18400.16486746089773915No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18100.16217940447005863No Hit
ATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGAGGCAGAGGGGCCAGACT18000.16128338566083178No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17760.15913294051868737No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17650.15814731982853786No Hit
CTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCC17430.15617607844823878No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17430.15617607844823878No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17230.1543840408297851No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17160.15375682766332632No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTT17040.1526816050922541No Hit
CCCCTGAGGGCCGCTGATCGTTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAA16830.15079996559287773No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG16500.14784310352242916No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGG16430.14721589035597035No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16370.14667827907043426No Hit
CTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCC16330.1463198715467435No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT16020.1435422132381403No Hit
ACCTACTACAACCCGTCCCTCAACAGTCGAGTCACCATATCCGGAGACACGTCCAAGAACCAGTTCTCCCTGAAG15950.1429150000716815No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAAT15680.14049574928676903No Hit
TTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTTCCGATGT15570.13951012859661951No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA15550.13933092483477413No Hit
GCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG15350.13753888721632046No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15300.13709087781170703No Hit
GTAGTAGGTGGTCCCACTAAAATACATATTCGCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC14930.1337756082175677No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14890.13341720069387697No Hit
GGCCATCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAGCATGGGATGACAACCTGAGTGGT14820.13278998752741816No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCCCTGGCCATCAGTGGCCTCCGG14690.13162516307542327No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT14600.13081874614711914No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14490.1298331254569696No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14230.1275034765529798No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14190.12714506902928907No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14060.12598024457729418No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG14010.12553223517268075No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTG13910.1246362163634539No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13830.12391940131607243No Hit
GCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13600.1218585580548507No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13350.11961851103178359No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13040.11684085272318037No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG12960.1161240376757989No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12890.1154968245093401No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12470.11173354551058737No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12390.11101673046320587No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT12370.11083752670136052No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG12330.11047911917766978No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGT12210.10940389660659755No Hit
ATCCCATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCAC12170.10904548908290683No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAAC12100.10841827591644804No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12030.10779106274998924No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12030.10779106274998924No Hit
CTGATGGCCAGGGAGGCAGAGGGGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATCG11950.10707424770260776No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCC11920.10680544205983972No Hit
CCCAGGAACGGCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTC11800.1057302194887675No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG11780.10555101572692215No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTT11780.10555101572692215No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG11750.10528221008415407No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11680.10465499691769531No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC11670.1045653950367726No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11550.10349017246570041No Hit
CTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCC11440.10250455177555086No Hit
GTGTATTATTGTGTGACATATAGTTCGAATATCGCAGCTCCCCGGGACCA11350.10169813484724671No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG11340.10160853296632402No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG11340.10160853296632402No Hit
CCCCAGTAGTGGTCCCGGGGAGCTGCGATATTCGAACTATATGTCACACA11240.1007125141570972No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11170.10008530099063839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2400.081.2215570
AACTGTA802.820499E-454.14770570
GCCAGAG21400.053.64164770
GTTAATA200.00742365651.63554446
CGTATGC1350.045.7137541
GGTATCA7750.045.6437571
GGCTATA306.7749346E-445.34303719
TGGTATC2200.044.829792
GTGGTAT2300.042.886431
ATCTCGT1200.042.72925637
ACGAGAC1550.041.6900822
TCGTATG1500.041.1052440
CTTATAC1800.039.6822741
GTATCAA21450.039.6426281
ATCTCCG1650.039.159910
GATTAAC350.001445269338.872431
GTCCTAG29950.037.9666770
AATCTCG1350.037.95762636
TTATACA1800.037.787562
TACACAT1800.037.787565
Produced by FastQC (version 0.11.5)