FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004683860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116048
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTA45710.40957019769759007No Hit
GTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGAGGCAGAGGGGCCAGACTTGGAGCCAGA41440.3713101945436038No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT37830.33896391553051486No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC37380.33493183088899403No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32030.2869948245953579No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCAT31600.2831419437156825No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30380.272210514243115No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT27440.24586756125184578No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG27330.2448819405616963No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA25040.22436310983040159No Hit
GTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGGGCTGGATCCG24340.21809097816581366No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT24110.21603013490459191No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGG24060.2155821254999785No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23500.21056442016830815No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22920.20536751107479248No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22500.20160423207603975No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA22420.20088741702865826No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA22080.197840953077287No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA22030.19739294367267357No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCG21290.19076240448439494No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20550.1841318652961163No Hit
ATAATACACAGCCGTGTCTGCGGCGGTAACAGATCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTCCG20500.18368385589150288No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG20050.17965177124998208No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG20000.17920376184536865No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19910.1783973449170645No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTAACAGATCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTCCG19830.17768052986968302No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG19620.17579889037030666No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19340.1732900377044715No Hit
GTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACC18640.1670179060398836No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18280.16379223832666695No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT18240.16343383080297622No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTT18180.1628962195174401No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG17540.15716169913838832No Hit
GTATTATTGTGTGACATATAGTTCGAATATCGCAGCTCCCCGGGACCACTACTGGGGCCAGGGAACCCTGGTCAC17360.15554886528178No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17240.1544736427107078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17190.15402563330609437No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT17150.15366722578240363No Hit
ATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATG16940.15178558628302724No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTTTATTGGTACCAGCAGCTCCCAGGAACGGCCCC16860.15106877123564577No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16220.145334250856594No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG16160.14479663957105787No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCATCAGTAGTACTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAA15530.13915172107292875No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15460.13852450790646997No Hit
CCCCTGAGGGCCGCTGATCGTTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAA15220.13637406276432557No Hit
CTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCC15090.13520923831233067No Hit
ACCTACTACAACCCGTCCCTCAACAGTCGAGTCACCATATCCGGAGACACGTCCAAGAACCAGTTCTCCCTGAA14980.13422361762218113No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14910.13359640445572235No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14790.13252118188465012No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14590.13072914426619642No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14520.13010193109973767No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGG14470.12965392169512424No Hit
GCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT14390.12893710664774274No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC14350.128578699124052No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTA14310.12822029160036127No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14230.1275034765529798No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13980.12526342952991268No Hit
TTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAATAAACAAAATTACTTCCGATGTTG13980.12526342952991268No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA13890.12445701260160853No Hit
CTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT13740.12311298438776827No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13700.12275457686407754No Hit
GGCCATCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAGCATGGGATGACAACCTGAGTGGTTG13650.12230656745946411No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13560.12150015053115995No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT13310.11926010350809284No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13230.11854328846071137No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13130.11764726965148452No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13040.11684085272318037No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGT12840.11504881510472668No Hit
GTAGTAGGTGGTCCCACTAAAATACATATTCGCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC12780.11451120381919058No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCTCCCTGGCCATCAGTGGCCTCCGGT12750.11424239817642251No Hit
GCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG12730.11406319441457716No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT12710.11388399065273179No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA12710.11388399065273179No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12590.11280876808165957No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGT12130.10868708155921608No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG11880.106447034536149No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT11580.10375897810846846No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11450.10259415365647356No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT11450.10259415365647356No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11350.10169813484724671No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCA11250.10080211603801988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)