FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120697
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGGAGTCTGTCCTGGTTTCTGGTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGGATAAAAGACT63960.570716259613437No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAAGCCGGGGTCCCT58610.5229781109434575No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50120.44722168436249937No Hit
ATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT44200.3943974151800174No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT39130.3491577116740743No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC38700.3453208137435899No Hit
ATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA34500.30784413628304524No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33640.30017034042207663No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30820.2750074284128538No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA30090.26849362494947343No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT30070.26831516458061366No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29060.259302915953197No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG28140.25109373898564913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT26710.23833382261217798No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT26510.2365492189235806No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26110.23298001154638584No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC26020.23217693988651705No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC24440.21807857074659787No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGCTTCCCGGGTAGATGCCCAGTAAATGAGCAGGTT23760.21201091820536683No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGAC23340.20826325045931238No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23230.20728171843058382No Hit
TTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT23050.2056755751108462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG22590.2015709866270723No Hit
GTCAGGGACCCCGGCTTCCCGGGTAGATGCCCAGTAAATGAGCAGGTTAGGAGTCTGTCCTGGTTTCTGGTGGT22090.19710947740557888No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC21980.1961279453768503No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21830.1947894926104023No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21470.191577205970927No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21250.18961414191346992No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATC21000.18738338730272322No Hit
CTTTTATCCAGCTCCAACAATAAGAACTACTTAGCTTGGTACCACCAGAAACCAGGACAGACTCCTAACCTGCTC19970.1781926783064468No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19830.17694345572442863No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC19340.1725711766873651No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19320.17239271631850536No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA19160.17096503336762747No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC18700.16686044488385354No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTGGGCTGTGCTCGCGGATGTGTCCCTGGT18260.16293431676893932No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18070.16123894326477184No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18050.1610604828959121No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17730.15820511699415632No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT17490.15606359256783947No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17050.15213746445292528No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATCCAGCTCCA17000.15169131353077592No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16810.14999594002660843No Hit
GTAAATGAGCAGGTTAGGAGTCTGTCCTGGTTTCTGGTGGTACCAAGCTA16790.1498174796577487No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16540.147586725047002No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15800.14098369139919176No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC15680.13991292918603332No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATCCAGCTCCAACAATAAGAACT15600.1391990877105944No Hit
GGATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG15510.13839601605072557No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15400.13741448402199702No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15360.13705756328427757No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15070.13446988793581138No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15010.13393450682923216No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC14970.13357758609151268No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14960.13348835590708283No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT14850.13250682387835427No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATCCAGCTCCAACA14770.13179298240291534No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT14730.13143606166519586No Hit
GTCTATTACTGTGTGAGAGAGAGATCACGTTTGGGGGAGTTACCCTTATA14670.13090068055861664No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGC13830.12340534506650773No Hit
GTCCTGGTTTCTGGTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGGATAAAAGACTCTGGCTGGACTTGC13570.12108536027133114No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTT13480.12028228861146234No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13290.11858691510729484No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13260.11831922455400523No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13120.11707000197198707No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG13090.11680231141869749No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13020.11617770012768838No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATG12820.11439309643909103No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12710.11341156441036249No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG12570.11216234182834432No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGCTTCCCGGGT12560.11207311164391445No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG12550.1119838814594846No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12510.11162696072176512No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGT12390.11055619850860669No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12390.11055619850860669No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12310.10984235703316776No Hit
GCTCATTTACTGGGCATCTACCCGGGAAGCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT12220.10903928537329893No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12220.10903928537329893No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC12180.10868236463557947No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12160.10850390426671974No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAAT12120.10814698352900026No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA12030.10734391186913145No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11970.10680853076255224No Hit
AACCAGGACAGACTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAA11960.10671930057812237No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11920.10636237984040289No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11900.10618391947154315No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC11900.10618391947154315No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC11630.10377470449193671No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11610.10359624412307698No Hit
GAATCGGTCAGGGACCCCGGCTTCCCGGGTAGATGCCCAGTAAATGAGCA11410.1018116404344796No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11390.10163318006561987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG28450.060.91270
GTGGTAT2900.055.6731831
TTGTGAT800.00745176851.57578770
TGGTATC3200.050.4290432
ACCCTGG9850.044.6815670
GGTATCA9650.041.4784471
CAGTTCG24250.040.26810570
GACAGGT1001.2732926E-1136.999471
TCGCGGG3852.6011548E-1035.7234970
TCACATG1550.002255982535.49301570
GTATCAA22400.033.9362031
CTGTGTT1700.003548048232.3612870
TCAAATG1700.003548048232.3612870
AAGTCAT2552.0745618E-532.3612870
CCACGTG6550.031.49666470
GTGCCCG4901.0186341E-1030.87530370
TGAATAT6250.030.80793870
GTCTAAG450.004598524430.69940446
TATCAAC24850.030.5753572
GTCCTTG36750.029.56544170
Produced by FastQC (version 0.11.5)