FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908150
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTGTTCTTGGAATTGTCTCTGG37700.4151296591972692No Hit
GATTATTACTGTGCAACATGGGATGACATCCTGAATGGTGTGGTCTTCGG28370.3123933270935418No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGACCCTCCGCCGAAGACCAC28150.30997081979849145No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27750.305566261080218No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAG25440.2801299344821891No Hit
CTATATTACTGTGCGAAAGATCTATACGGAATACGGTCCGCCGGGGGTTT24710.2720916148213401No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC24260.26713648626328246No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22850.25161041678136875No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA20940.23057864890161317No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20480.22551340637559872No Hit
ATATTACTGTGCGAAAGATCTATACGGAATACGGTCCGCCGGGGGTTTTGACTACTGGGGCCAGGGAACCCTGGT20460.22529317843968505No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19690.21681440290700874No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG18870.20778505753454826No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18780.2067940318229367No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17370.19126796234102295No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16910.18620271981500852No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC16510.1817981610967351No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGACCCTC16510.1817981610967351No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA16360.1801464515773826No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15510.1707867643010516No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14930.16440015415955514No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14690.16175741892859108No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14380.1583438859219292No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14360.15812365798601552No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC14290.1573528602103177No Hit
AGCACATACTACGCAGACTCCGTGAAGGCCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTG14040.15460001101139678No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT13990.15404944117161262No Hit
ATTCAGGATGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGG13990.15404944117161262No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13330.14678191928646148No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13190.1452403237350658No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12960.14270770247205858No Hit
CCCTGAGACTCTCCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCC12890.14193690469636072No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12860.1416065627924902No Hit
GCCCTATATTACTGTGCGAAAGATCTATACGGAATACGGTCCGCCGGGGGTTTTGACTACTGGGGCCAGGGAACC12810.14105599295270607No Hit
GTATGTGCTACCACCACTTCCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT12810.14105599295270607No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT12690.13973462533722403No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG12600.1387435996256125No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12590.13863348565765568No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGCAAAGCTGCTAAAGGTGAATCCAGAGGGTTCAC12540.1380829158178715No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC12410.13665143423443263No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC12390.13643120629851896No Hit
CCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGG12370.1362109783626053No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12360.13610086439464847No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12330.13577052249077795No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12260.1349997247150801No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12080.13301767329185707No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12050.13268733138798655No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11910.1311457358365909No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC11740.1292737983813247No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAATTGGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTT11700.12883334250949732No Hit
GTGTGAGGTGCAGCTGGTGGCGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAACC11540.12707151902218797No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11480.12641083521444696No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11300.12442878379122391No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAACCCCCGGCGG11190.12321753014369873No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11120.12244673236800087No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11100.1222265044320872No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGCGT11020.12134559268843254No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10970.12079502284864836No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10940.12046468094477786No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCCATGACCTGGGTCCG10860.11958376920112317No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10720.11804217364972747No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC10680.11760171777790013No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAACCCCCGGCGGACCGTATTCCGTATAGATCTTTCGCACAGTAATATAGGG10670.11749160380994329No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA10600.11672080603424545No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG10570.11639046413037493No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10270.11308704509166986No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCC10260.11297693112371303No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACATCCTGAATGGTGTGGTCTTCGGCGGAGGGTC10260.11297693112371303No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10210.11242636128392886No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACC10180.11209601938005836No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC10170.11198590541210153No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC10130.11154544954027419No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10100.11121510763640369No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCCATG10090.11110499366844685No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC10090.11110499366844685No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC10080.11099487970049002No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT10060.11077465176457635No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG10040.11055442382866268No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC10010.11022408192479216No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC9930.10934317018113748No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC9930.10934317018113748No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9760.10747123272587128No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG9670.10648020701425975No Hit
GATTATTACTATAGATGAGGAATTGGGGGGCCGTTCCTGGGAGCTGCTGG9590.10559929527060508No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9580.10548918130264824No Hit
CTCCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG9540.10504872543082089No Hit
CTACGCAGACTCCGTGAAGGCCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGA9520.10482849749490722No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTC9480.10438804162307988No Hit
GACCGAGGACGGTCAGCTTGGACCCTCCGCCGAAGACCACACCATTCAGGATGTCATCCCATGTTGCACAGTAAT9390.10339701591146835No Hit
GCTTGGACCCTCCGCCGAAGACCACACCATTCAGGATGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCT9380.10328690194351152No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC9290.10229587623190002No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9270.10207564829598635No Hit
GTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCC9260.10196553432802952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9150.10075428068050434No Hit
CTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTC9130.10053405274459065No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCG9090.10009359687276331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATG701.07239895E-465.8392270
GTTAAAT600.004810328657.60931870
TGGTATC1850.057.183992
GTGGTAT1950.054.2544671
TAAAGCG200.007451123651.58656744
ACTCTAG200.00768095451.1917151
GAGCCAT10650.047.60206270
GCTTAGT306.6572067E-445.5037461
CATGTTG16850.043.7625770
GGTATCA5750.042.733951
ACCCTGG15400.041.89768270
GTATCAA12850.036.1197051
ACTGGTG19950.035.22975570
AGGGGGG1651.7316572E-434.91473870
ATGCCGT400.002636618434.39104544
TCTCGCG12100.034.27992270
TCTCGTA400.002680673534.27502438
GCAGCGT400.002737853234.1278081
GCATGCA400.00273933234.124053
GGTTATC400.00273933234.124057
Produced by FastQC (version 0.11.5)