FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908150
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTGTTCTTGGAATTGTCTCTGG35260.38826185101580135No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28850.3176787975554699No Hit
GATTATTACTGTGCAACATGGGATGACATCCTGAATGGTGTGGTCTTCGG27240.29995044871441945No Hit
CTATATTACTGTGCGAAAGATCTATACGGAATACGGTCCGCCGGGGGTTT22630.24918790948631836No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC20300.22353135495237572No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGACCCTCCGCCGAAGACCAC20170.22209987336893686No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19980.22000770797775698No Hit
ATATTACTGTGCGAAAGATCTATACGGAATACGGTCCGCCGGGGGTTTTGACTACTGGGGCCAGGGAACCCTGGT19660.21648406100313825No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18190.20029730771348345No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG17800.19600286296316688No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA17640.19424103947585752No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16810.18510158013544017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15950.17563177889115233No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15840.17442052524362714No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA15600.1717777900126631No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT15170.1670428893905192No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14860.16362935638385728No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGCAAAGCTGCTAAAGGTGAATCCAGAGGGTTCAC14690.16175741892859108No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14450.15911468369762705No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC14400.15856411385784286No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC13790.1518471618124759No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13700.1508561361008644No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC13680.15063590816495073No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13670.1505257941969939No Hit
GCCCTATATTACTGTGCGAAAGATCTATACGGAATACGGTCCGCCGGGGGTTTTGACTACTGGGGCCAGGGAACC13530.1489841986455982No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13430.14788305896602985No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGACCCTC13300.14645157738259099No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13210.14546055167097946No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12970.1428178164400154No Hit
AGCACATACTACGCAGACTCCGTGAAGGCCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTG12870.14171667676044705No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT12860.1416065627924902No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT12500.13764245994604415No Hit
GTATGTGCTACCACCACTTCCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT11860.1305951659968067No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC11680.12861311457358365No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11670.12850300060562683No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCC11560.12729174695810164No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11560.12729174695810164No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAACCCCCGGCGGACCGTATTCCGTATAGATCTTTCGCACAGTAATATAGGG11540.12707151902218797No Hit
CCCTGAGACTCTCCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCC11370.12519958156692176No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACATCCTGAATGGTGTGGTCTTCGGCGGAGGGTC11360.12508946759896492No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG11350.1249793536310081No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG11270.1240984418873534No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11210.1234377580796124No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAATTGGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTT11190.12321753014369873No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11060.12178604856025987No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11040.1215658206243462No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10780.11870285745746849No Hit
CCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGG10740.11826240158564114No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAACCCCCGGCGG10730.1181522876176843No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT10610.11683092000220228No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10550.11617023619446126No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG10540.11606012222650443No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10450.11506909651489292No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAG10430.11484886857897923No Hit
GTGTGAGGTGCAGCTGGTGGCGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAACC10350.11396795683532455No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10170.11198590541210153No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10150.11176567747618786No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10120.11143533557231736No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCCATGACCTGGGTCCG10090.11110499366844685No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10000.11011396795683533No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC9960.10967351208500799No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC9910.10912294224522381No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9830.10824203050156912No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGCGT9820.10813191653361229No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATC9720.10703077685404394No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9620.10592963717447558No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC9610.10581952320651875No Hit
GGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAACCCTCTGGATTCACCTTTAGCAGCTTTGCCATG9520.10482849749490722No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA9510.10471838352695038No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA9470.10427792765512305No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9420.10372735781533887No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9410.10361724384738204No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9140.1006441667125475No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTC9140.1006441667125475No Hit
GCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG9130.10053405274459065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1450.056.435461
TGGTATC1600.051.0910872
GGTATCA5500.045.875191
CATGTTG13050.041.8729270
ACTGGTG17850.039.6577270
ATAGCGG459.175643E-538.90651363
ACCCTGG15500.038.45923670
GTATCAA14600.038.3000951
GTGCTAG451.0732356E-437.884921
GTGGCCG12250.035.48322370
TATCAAC15900.035.1318172
CGCGTCT400.00277111834.04384216
ATCAACG17400.032.080233
GCGCGTG7750.032.04936270
CCACGTG8600.031.76986370
AATAGCG553.0218464E-431.76601862
TCAACGC17600.031.7139344
ACGAACC553.2331393E-431.40209452
CAAACGA553.2682644E-431.3442949
ACAAACG553.2778986E-431.3285648
Produced by FastQC (version 0.11.5)