FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646438
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGTACTTTGGTAACTCACCTCCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGA18140.28061469158681884No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17740.2744269365352903No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT16490.2550902019992637No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGCGGTTATTACTGGAACTGGATCCG15030.23250489606118455No Hit
CAGTAATACACCGCAAAATCTTCAGGCTCTAGTCTGCTGATCGTGAGAGTGAAGTCTGTCTCAGACCCACCGCC14620.22616244713336778No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC13860.21440571253546356No Hit
CAATAATACACGGCCGTGTCCGCGGCACTCACAGACTTCAGCATCAGGGAGAACTGGTTCTTAGACGTGTCTT13160.20357714119528864No Hit
ATAATACACGGCCGTGTCCGCGGCACTCACAGACTTCAGCATCAGGGAGAACTGGTTCTTAGACGTGTCTTCTG11760.18191999851493879No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11740.18161061076236237No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11680.18068244750463308No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT11500.17789795773144526No Hit
GTCTGAGACAGACTTCACTCTCACGATCAGCAGACTAGAGCCTGAAGATT11180.17294775369022242No Hit
GTGTTGGCAGCGACTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGACTCCTCATCTATGGCGCAT10750.16629591700982926No Hit
GTGTATTACTGTCAGTACTTTGGTAACTCACCTCCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGA10550.16320203948406498No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10360.16026285583458894No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG10300.15933469257685967No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9630.14897020286554935No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9360.1447934682057676No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGCGGTTATTACTGGAACTGGATCCGCCAGCACCCAGGGA9270.14340122331917368No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9090.14061673354598586No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG8910.137832243772798No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8870.13721346826764516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA8850.13690408051506875No Hit
GTATTATTGTGCGAGAGGCCACCCCCTACGAAACAGGGGGGGTCTGGACGTCTGGGGCCAGGGGACCACGGTCAC8520.13179918259755768No Hit
GTAATACACCGCAAAATCTTCAGGCTCTAGTCTGCTGATCGTGAGAGTGAAGTCTGTCTCAGACCCACCGCC8450.1307163254635402No Hit
GTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGTTCCAGTAATAACCGCC8390.12978816220581094No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTGTTGGC8180.12653959080375846No Hit
CTCCTGCAGGGCCAGTCACAGTGTTGGCAGCGACTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCC8140.12592081529860558No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8060.12468326428829989No Hit
GTCTCAGACCCACCGCCACTGAACTTGTCTGGGATGCCAGTGGCCCTGCT7960.12313632552541774No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG7950.12298163164912954No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7910.12236285614397668No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7790.12050652962851813No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG7650.11834081536048315No Hit
TAGCAGGGCCACTGGCATCCCAGACAAGTTCAGTGGCGGTGGGTCTGAGACAGACTTCACTCTCACGATCAGC7550.11679387659760102No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCACAGTGTTGGCAGCG7360.11385469294812496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA7340.11354530519554853No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCC7330.11339061131926031No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7300.11292652969039567No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC7290.11277183581410748No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7290.11277183581410748No Hit
ACTACAACCCGTCCCTGAAGAGTCGACTTACCATGTCAGAAGACACGTCTAAGAACCAGTTCTCCCTGATGCTGA7260.11230775418524283No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6950.1075122440203082No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCCTGG6940.10735755014401999No Hit
GTCACAGTGTTGGCAGCGACTACTTAGCCTGGTACCAGCAGAGACCTGGC6840.10581061138113786No Hit
GGCCAAAAGTGTACGGAGGTGAGTTACCAAAGTACTGACAGTAATACACCGCAAAATCTTCAGGCTCTAGTCTG6830.10565591750484966No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6780.10488244812340858No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCC6710.1037995909893911No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCACA6700.10364489711310289No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTGTACGGAGGTGAGTTACCAAAGT6690.10349020323681467No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6690.10349020323681467No Hit
CTCCCAGACTCCTCATCTATGGCGCATATAGCAGGGCCACTGGCATCCCAGACAAGTTCAGTGGCGGTGGGTCTG6660.10302612160795002No Hit
CCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC6620.10240734610279718No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6570.1016338767213561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT150.0036981748180.2123670
CAATGCG150.0036981748180.2123670
GAACGTT200.008741795135.1592770
TGACTAG150.00235404369.00741646
GAGCCAT4600.055.8266670
ATGCATG750.006180431754.0637170
GTACTAT252.9734915E-453.578461
GGTATCA5350.050.699241
TACATTA200.00828047750.2259182
ATGCCGT1350.045.81136744
AGTCTGG5750.044.66132470
CGTATGC1400.043.9311141
ACGAGAC1450.043.88909522
CTGGAGT10800.043.80161770
TCGTATG1450.042.32601540
ACGGGTA405.9692367E-541.84845416
AGACCGA1600.039.81455225
CCCACGA1600.039.77449419
AAAGGGG1700.038.91813368
GCAGAGG7300.038.88143570
Produced by FastQC (version 0.11.5)