FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646438
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGTACTTTGGTAACTCACCTCCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGA17230.26653754884459147No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16680.25802938564873973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15810.24457101841166515No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGCGGTTATTACTGGAACTGGATCCG14640.2264718348859442No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT13730.2123946921437168No Hit
CAGTAATACACCGCAAAATCTTCAGGCTCTAGTCTGCTGATCGTGAGAGTGAAGTCTGTCTCAGACCCACCGCC12930.20001918204065974No Hit
CAATAATACACGGCCGTGTCCGCGGCACTCACAGACTTCAGCATCAGGGAGAACTGGTTCTTAGACGTGTCTT12110.18733428418502626No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC11500.17789795773144526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11410.17650571284485134No Hit
ATAATACACGGCCGTGTCCGCGGCACTCACAGACTTCAGCATCAGGGAGAACTGGTTCTTAGACGTGTCTTCTG10750.16629591700982926No Hit
GTCTGAGACAGACTTCACTCTCACGATCAGCAGACTAGAGCCTGAAGATT10650.16474897824694712No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCACCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC10640.1645942843706589No Hit
GTGTTGGCAGCGACTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGACTCCTCATCTATGGCGCAT9860.15252816202017827No Hit
GTGTATTACTGTCAGTACTTTGGTAACTCACCTCCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGA9840.15221877426760186No Hit
GTCTCAGACCCACCGCCACTGAACTTGTCTGGGATGCCAGTGGCCCTGCT9630.14897020286554935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9560.14788734573153187No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA9540.14757795797895545No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9510.1471138763500908No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG9050.13999795804083298No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8860.13705877439135694No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8790.13597591725733946No Hit
GTAATACACCGCAAAATCTTCAGGCTCTAGTCTGCTGATCGTGAGAGTGAAGTCTGTCTCAGACCCACCGCC8560.13241795810271056No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8160.12623020305118202No Hit
GTATTATTGTGCGAGAGGCCACCCCCTACGAAACAGGGGGGGTCTGGACGTCTGGGGCCAGGGGACCACGGTCAC8150.1260755091748938No Hit
GTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGTTCCAGTAATAACCGCC8130.12576612142231736No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGCGGTTATTACTGGAACTGGATCCGCCAGCACCCAGGGA8130.12576612142231736No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7850.12143469288624739No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7840.1212799990099592No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7390.11431877457698958No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTGTACGGAGGTGAGTTACCAAAGT7260.11230775418524283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7240.11199836643266639No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7240.11199836643266639No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG7220.11168897868008996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC6980.10797632564917285No Hit
CTCCTGCAGGGCCAGTCACAGTGTTGGCAGCGACTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCC6940.10735755014401999No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC6920.10704816239144359No Hit
GGCCAAAAGTGTACGGAGGTGAGTTACCAAAGTACTGACAGTAATACACCGCAAAATCTTCAGGCTCTAGTCTG6860.10611999913371428No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA6800.10519183587598502No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6700.10364489711310289No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6660.10302612160795002No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6630.10256203997908539No Hit
GTCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTAAGTAGTCGCTGCCAACACTGTGACTGGCCCTGCAGG6610.10225265222650896No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC6550.10132448896877969No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6530.10101510121620326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA200.004331261459.16452867
GAAAGTT1103.4574413E-451.94089570
ATCTAGG200.0083238750.1595341
ATAAACG200.00835192450.1168563
CAGTTCG7150.049.94317270
GGTATCA5350.048.128161
GTGGCCG7250.045.31395370
CTGGAGT9500.042.0994670
ATAGTTT359.864088E-442.00887764
ATTCCGG4000.039.28030470
AGTCTGG8500.038.65013570
CAACTTT1500.00159276438.0899970
TATCTGG5300.037.7306570
TCTTGTT4201.0913936E-1137.40981370
TCTCGCG3452.415618E-937.2619570
GAGCCAT5400.037.03193370
GTATCAA14050.035.4627341
ACTCAGT5253.6379788E-1232.64856370
TATCAAC15200.032.1023062
GCGACCT450.00411541731.40157955
Produced by FastQC (version 0.11.5)