FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004683877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616515
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35060.5686804051807336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT32860.5329959530587253No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGG23410.37971501098918925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23190.3761465657769884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22320.36203498698328507No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT22230.36057516848738475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG20860.3383534869386795No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC20600.3341362335060785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19040.3088327129104726No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18670.30283123687177116No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18280.2965053567228697No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC18200.29520774028206936No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG17700.28709763752706746No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16520.267957795025263No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15660.2540084182866597No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15410.24995336690915873No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15120.2452495073112576No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCTAGTTTGT14640.2374638086664558No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA14570.23632839428075555No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCTAGTTTGTAGAGCTTGCATGCAGT14380.23324655523385482No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14330.23243554495835464No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14000.22708287714005337No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13770.2233522298727525No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCC13440.2179995620544512No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC13410.21751295588915112No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC13280.21540432917285063No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATG13240.21475552095245048No Hit
CTACAAACTAGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT13030.21134927779534965No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT13020.2111870757402496No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGT12980.21053826751984947No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG12790.20745642847294876No Hit
AGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT12720.20632101408724848No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC12570.2038879832607479No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT12390.20096834626894722No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTAGGACTTTCGGCGGAGGGAC12380.20080614421384718No Hit
TTATTACTGCATGCAAGCTCTACAAACTAGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG11950.19383145584454556No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC11780.1910740209078449No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11460.18588355514464366No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11140.18069308938144246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11140.18069308938144246No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAT10990.1782600585549419No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC10860.17615143183864138No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10390.1685279352489396No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGG10380.16836573319383957No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10300.16706811675303926No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC10240.16609490442243904No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTAATGG10230.165932702367339No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10090.16366187359593845No Hit
AACTAGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG9880.16025563043883767No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9810.1591202160531374No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC9800.15895801399803736No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC9770.15847140783273725No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9730.15782259961233708No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTAGGACTTTCGGCGGAGGGACCA9530.1545785585103363No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9480.15376754823483613No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT9440.15311874001443598No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT9430.15295653795933595No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9420.1527943359042359No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAAT9300.15084791124303545No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9100.14760387014103468No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9040.14663065781043447No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT9000.1459818495900343No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCAC8990.14581964753493426No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT8930.14484643520433405No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC8760.14208900026763338No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC8740.1417645961574333No Hit
GTCCTAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTA8630.1399803735513329No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8530.13835835300033253No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8300.13462770573303165No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8270.13414109956773151No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGG8070.13089705846573077No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8040.13041045230043063No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT8020.13008604819023056No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC8000.1297616440800305No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7980.12943723996983042No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7930.1286262296943302No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA7920.1284640276392302No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT7850.12732861325352993No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC7850.12732861325352993No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC7850.12732861325352993No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGT7820.1268420070882298No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA7760.12586879475762958No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7670.12440897626172923No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC7650.12408457215152915No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7560.1226247536556288No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT7500.12165154132502858No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7500.12165154132502858No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7460.12100273310462843No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCT7450.12084053104952841No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7450.12084053104952841No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC7420.12035392488422829No Hit
GTAATACACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTCGCGGATGTGTCCCTGGT7350.11921851049852801No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7330.11889410638832792No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT7300.11840750022302782No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT7280.11808309611282776No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTG7250.11759648994752764No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7240.11743428789242759No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7160.1161366714516273No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC7130.1156500652863272No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6950.11273042829452648No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG6860.11127060979862614No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6780.10997299335782583No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC6750.10948638719252572No Hit
CCTTGGTCCCTCCGCCGAAAGTCCTAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCC6730.10916198308232566No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGG6730.10916198308232566No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGC6720.1089997810272256No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT6420.10413371937422447No Hit
GGTTTATTACTGCATGCAAGCTCTACAAACTAGGACTTTCGGCGGAGGGA6410.10397151731912443No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATC6350.10299830498852422No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG6330.10267390087832413No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC6310.10234949676812405No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6310.10234949676812405No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT6230.10105188032732373No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGCAGGAGGCTCTGACT6180.10024087005182357No Hit
ACCTTGGTCCCTCCGCCGAAAGTCCTAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCC6180.10024087005182357No Hit
TTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC6170.1000786679967235No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG6170.1000786679967235No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTG6170.1000786679967235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)