FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004683881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909673
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45560.5008393125881497No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT41000.45071140948450705No Hit
ACTTTAGAGAGTGGAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGT38260.42059069577749364No Hit
GTGTCATATTGTTGACAGTAATAAGTTGCAAAGTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC33440.36760462276004674No Hit
ATATGACACTGACCCCGCCTTTGGCCCTGGGACTAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT32000.3517747586220543No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC30310.3331966541823271No Hit
CTCTAAAGTCGACGCCTTATAGATCAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCATCT29980.32956897698403714No Hit
GAGTATTAGTAGATGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCC29400.3231930594840124No Hit
GTATTAGTAGATGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTGATCTATAAGGCGTCG26510.29142340159595814No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26140.2873560059493906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25770.2832886103028231No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25260.2776822000872841No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25070.27559353745796566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24090.26482043547516526No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23680.2603133213803202No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22370.2459125421992298No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTAGTCCCAGGGCCAAAGGCGGGGTCAGTGTCATATTGTTGACAGT21310.23426000331987432No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC19630.21579182849221645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC19530.21469253237152253No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG19370.21293365857841226No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT18810.20677760030252632No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18480.20314992310423635No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17380.19105766577660324No Hit
ACCCAGTCCCCTTCCATCTTGTCTGCATCTGTGGGAGACAGAATCACCGTCACTTGCCGGGCCAGTCAGAGTATT16920.18600090362141122No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16740.18402217060416215No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16330.17951505650931707No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC15560.1710504763799739No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT15300.1681923064661697No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14810.1628057554747695No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGACTTTGCAACTTATTACTGTCAACAATATGACACTGAC14740.16203624819028378No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14720.16181638896614498No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14710.1617064593540756No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTAGTCCCAGGGCCAAAGGCGGGGTCAGTGTC14620.16071709284545105No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC14620.16071709284545105No Hit
GATCTATAAGGCGTCGACTTTAGAGAGTGGAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTC14600.16049723362131227No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14390.15818871176785504No Hit
CTTTAGTCCCAGGGCCAAAGGCGGGGTCAGTGTCATATTGTTGACAGTAATAAGTTGCAAAGTCATCAGGCTGC14070.15467096418163448No Hit
ACTTATTACTGTCAACAATATGACACTGACCCCGCCTTTGGCCCTGGGAC14020.15412131612128754No Hit
GTCCCCTTCCATCTTGTCTGCATCTGTGGGAGACAGAATCACCGTCACTTGCCGGGCCAGTCAGAGTATTAGT13980.15368159767300998No Hit
CTACTAATACTCTGACTGGCCCGGCAAGTGACGGTGATTCTGTCTCCCACAGATGCAGACAAGATGGAAGGGG13940.1532418792247324No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13790.15159293504369153No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13730.15093335737127517No Hit
GTAATAAGTTGCAAAGTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC13570.1491744835781649No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13430.14763546900919342No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13180.14488722870745863No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC13070.1436780029746953No Hit
CCTTTGGCCCTGGGACTAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG13070.1436780029746953No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12820.1409297626729605No Hit
GTCTGCAGCCTGATGACTTTGCAACTTATTACTGTCAACAATATGACACTGACCCCGCCTTTGGCCCTGGGACTA12780.14049004422468295No Hit
GGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTGATCTATAAGGCGTCGACTTTAGAGAGTGGAGTCCCAT12540.13785173353501753No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12290.13510349323328275No Hit
TCTATAAGGCGTCGACTTTAGAGAGTGGAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTCACT11890.13070630875050704No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11810.12982687185395192No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11750.12916729418153555No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11670.12828785728498043No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCATCTACTAATACTCTG11430.12564954659531502No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11350.12477010969875989No Hit
CACCATCAGCAGTCTGCAGCCTGATGACTTTGCAACTTATTACTGTCAACAATATGACACTGACCCCGCCTTTGG11310.12433039125048231No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11160.12268144706944144No Hit
CTAATACTCTGACTGGCCCGGCAAGTGACGGTGATTCTGTCTCCCACAGA11130.12235165823323325No Hit
GGACTAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA11040.12136229172460875No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11000.12092257327633116No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACTCCACTCTCTAAAGTCGACGCCTTAT10970.12059278444012297No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10850.11927362909529028No Hit
GTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGG10520.11564595189700036No Hit
TTATAGATCAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGC10430.11465658538837582No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10310.11333743004354313No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG10300.11322750043147373No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATG10270.11289771159526554No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10260.11278778198319617No Hit
GGCCAAAGGCGGGGTCAGTGTCATATTGTTGACAGTAATAAGTTGCAAAG10240.11256792275905739No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCCCCTTCCATCTTGTCTGCATCTGTGGG10230.11245799314698798No Hit
GTCATATTGTTGACAGTAATAAGTTGCAAAGTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCC10150.11157855625043285No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTC9850.10828066788835108No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC9790.10762109021593474No Hit
GGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACTCCACT9730.10696151254351838No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9680.10641186448317143No Hit
GTGGAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTG9300.10223453922453454No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9210.10124517271591No Hit
CTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCT9140.10047566543142426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)