FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909673
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45130.4961123392691659No Hit
ATATGACACTGACCCCGCCTTTGGCCCTGGGACTAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT36230.3982749845274071No Hit
GTGTCATATTGTTGACAGTAATAAGTTGCAAAGTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC34920.3838742053463168No Hit
ACTTTAGAGAGTGGAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGT34520.37947702086354107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT33870.3723315960790306No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC28800.3165972827598489No Hit
CTCTAAAGTCGACGCCTTATAGATCAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCATCT28410.3123100278891426No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27120.298129107932191No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25230.2773524112510759No Hit
GAGTATTAGTAGATGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCC25080.27570346707003507No Hit
GTATTAGTAGATGGTTGGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTGATCTATAAGGCGTCG24300.26712895732862246No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24250.2665793092682755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23640.25987360293204265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22700.24954021939751977No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTAGTCCCAGGGCCAAAGGCGGGGTCAGTGTCATATTGTTGACAGT21360.23480965138022125No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19930.21908971685429818No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18240.20051161241457097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG18200.20007189396629338No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17990.19776337211283612No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC17950.1973236536645586No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17950.1973236536645586No Hit
ACCCAGTCCCCTTCCATCTTGTCTGCATCTGTGGGAGACAGAATCACCGTCACTTGCCGGGCCAGTCAGAGTATT17080.1877597774145215No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT16870.18545125556106426No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16430.18061435263001102No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT16180.1778661123282762No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15660.17214977250066782No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15480.17017103948341877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14610.16060716323338167No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC14480.1591780782764796No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14240.15653976758681418No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14100.15500075301784266No Hit
GATCTATAAGGCGTCGACTTTAGAGAGTGGAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTC13880.15258230155231606No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGACTTTGCAACTTATTACTGTCAACAATATGACACTGAC13620.14972413163851186No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA13550.14895462435402612No Hit
GTCCCCTTCCATCTTGTCTGCATCTGTGGGAGACAGAATCACCGTCACTTGCCGGGCCAGTCAGAGTATTAGT13490.14829504668160978No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13380.14708582094884645No Hit
CTACTAATACTCTGACTGGCCCGGCAAGTGACGGTGATTCTGTCTCCCACAGATGCAGACAAGATGGAAGGGG13350.14675603211263827No Hit
GTAATAAGTTGCAAAGTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC13170.14477729909538922No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13090.14389786219883408No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTAGTCCCAGGGCCAAAGGCGGGGTCAGTGTC13000.14290849569020955No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC12950.1423588476298626No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12830.14103969228502988No Hit
ACTTATTACTGTCAACAATATGACACTGACCCCGCCTTTGGCCCTGGGAC12830.14103969228502988No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC12800.14070990344882173No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12760.14027018500054417No Hit
CTTTAGTCCCAGGGCCAAAGGCGGGGTCAGTGTCATATTGTTGACAGTAATAAGTTGCAAAGTCATCAGGCTGC12610.13862124081950325No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC12230.13444391556086638No Hit
GTCTGCAGCCTGATGACTTTGCAACTTATTACTGTCAACAATATGACACTGACCCCGCCTTTGGCCCTGGGACTA12010.13202546409533975No Hit
CCTTTGGCCCTGGGACTAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG12000.13191553448327037No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11860.13037651991429888No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11550.12696870194014773No Hit
TCTATAAGGCGTCGACTTTAGAGAGTGGAGTCCCATCAAGATTCAGTGGCAGTGGATCTGGGACAGAATTCACT11520.12663891310393954No Hit
GGTATCAGCAGAAACCAGGAAAAGCCCCTAAACTCCTGATCTATAAGGCGTCGACTTTAGAGAGTGGAGTCCCAT11470.12608926504359258No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11410.12542968737117624No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACTCCACTCTCTAAAGTCGACGCCTTAT11290.12411053202634353No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10910.11993320676770665No Hit
GGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACTCCACT10770.11839419219873515No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10680.11740482569011063No Hit
GTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGG10490.11531616306079218No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10470.1150963038366534No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10370.11399700771595948No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCCAACCATCTACTAATACTCTG10110.11113883780215526No Hit
GGACTAAAGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA10050.11047926012973894No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10000.10992961206939197No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9990.10981968245732257No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9820.10795087905214291No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9790.10762109021593474No Hit
GGCCAAAGGCGGGGTCAGTGTCATATTGTTGACAGTAATAAGTTGCAAAG9770.10740123099179597No Hit
GTGGGAGACAGAATCACCGTCACTTGCCGGGCCAGTCAGAGTATTAGTAGATGGTTGGCCTGGTATCAGCAGAA9760.10729130137972656No Hit
CACCATCAGCAGTCTGCAGCCTGATGACTTTGCAACTTATTACTGTCAACAATATGACACTGACCCCGCCTTTGG9670.10630193487110202No Hit
GTCATATTGTTGACAGTAATAAGTTGCAAAGTCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCC9470.10410334262971419No Hit
CTAATACTCTGACTGGCCCGGCAAGTGACGGTGATTCTGTCTCCCACAGA9460.1039934130176448No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC9450.10388348340557542No Hit
CAATATGACACTGACCCCGCCTTTGGCCCTGGGACTAAAGTGGAGATCAAACGAACTGTGGCTGCACCAT9450.10388348340557542No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9400.10333383534522846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9390.10322390573315905No Hit
CTCCTGATCTATAAGGCGTCGACTTTAGAGAGTGGAGTCCCATCAAGATT9370.10300404650902027No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC9280.10201468000039574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATTT800.006989263552.41494870
CAGTTCG19000.044.138970
GTGGTAT2450.042.792741
GTCACCG4550.039.93520470
AAGCGGT5150.035.28255570
CGTCGTA400.002608956534.46505444
GTGCCCG3359.4252755E-833.37867770
GTATCAA21100.032.858361
GGCGTCG23100.032.06917670
CACAGGT1800.00433521431.0607170
ATCAACG21500.030.9460373
TCGTAGG450.004585506430.71658546
TCTCGCG3202.4878918E-630.57538670
TCAACGC21800.030.5201764
AATAGTC902.1952292E-730.33275236
CAACGCA22050.030.1774585
AACGCAG22400.029.707576
TATCAAC23950.028.6390652
CAGCCAT7850.028.48880670
ACCCATA500.007522401427.75732250
Produced by FastQC (version 0.11.5)