FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004683885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244793
Sequences flagged as poor quality0
Sequence length21-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC8830.36071292888277035No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA8070.3296662894772318No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7820.31945357914646255No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGACCTTTCATGTGAAGATACAGAGTGTTCTTCGAATTGTCTCTGG7760.3170025286670779No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6590.26920704431907777No Hit
CTCTTTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT5410.22100305155784683No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA5090.2079307823344622No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC5020.20507122344184678No Hit
CTCCTCACTCTTTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGG4980.2034371897889237No Hit
AACCAGGACAGGCCCCTTTACTTGTCGTCTATTATACAAACAGCCGGCCC4850.19812658041692366No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC4590.18750536167292364No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC4530.18505431119353902No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC4420.18056071864800055No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC4280.17484160086276979No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG4230.17279905879661592No Hit
GTGTATTACTGTGCGAGAACGCCCGCGGACCCTAATTGTAGTTATTCCAA4200.1715735335569236No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGGGACAGCCTCAGAAGG4150.16953099149076975No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT4060.16585441577169283No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGGTGGAGCC3960.16176933163938512No Hit
GTGTGAGGTGCAGCTTATGGAGTCTGGAGGAGGCTTGATCCAGCCTGGGGGGTCCCTGAGACTTTCCTGCGCAGC3950.16136082322615433No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3880.15850126433353895No Hit
GTCCTGGTTTCTGCTGGTACCAGCTTGCATAATGCCTTCTGAGGCTGTCCCCTTGGCATGTGATCCTGACTGT3840.15686723068061587No Hit
ACTACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCGAAGAACACTCTGTATCTT3840.15686723068061587No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTGACCGTTGGGTGTTCGGCG3800.15523319702769278No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3740.15278214654830818No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG3660.149514079242462No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGG3620.1478800455895389No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC3610.14747153717630815No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT3600.14706302876307736No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3540.14461197828369274No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGACCTTTCATGTGAAGATA3520.1437949614572312No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC3490.1425694362175389No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTGACCGTTGGGTGTTCGG3490.1425694362175389No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA3440.14052689415138506No Hit
GTAAAGGGGCCTGTCCTGGTTTCTGCTGGTACCAGCTTGCATAATGCCTT3430.14011838573815427No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG3410.13930136891169273No Hit
ATTATACAAACAGCCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCACCTCAGGAAACACAGCTTCCTTG3390.1384843520852312No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG3380.13807584367200043No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGGTGGAGCCAG3270.13358225112646196No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT3260.13317374271323118No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGGGACAGCCTCAGAAGGCATTATGCAAGCTGGT3250.13276523430000042No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3230.13194821747353888No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3150.1286801501676927No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG3130.12786313334123114No Hit
GTATTACTGTGCGAGAACGCCCGCGGACCCTAATTGTAGTTATTCCAACT3100.12663760810153885No Hit
CTATTATACAAACAGCCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCT3050.12459506603538499No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG3050.12459506603538499No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC3040.12418655762215422No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG3040.12418655762215422No Hit
GTATAATAGACGACAAGTAAAGGGGCCTGTCCTGGTTTCTGCTGGTACCAGCTTGCATAATGCCTTCTGAGG3020.12336954079569268No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA2950.1205099819030773No Hit
GCATTATGCAAGCTGGTACCAGCAGAAACCAGGACAGGCCCCTTTACTTGTCGTCTATTATACAAACAGCCGGCC2930.11969296507661575No Hit
GCCTCAGAAGGCATTATGCAAGCTGGTACCAGCAGAAACCAGGACAGGCCCCTTTACTTGTCGTCTATTATACAA2920.11928445666338498No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGCTCTGTGGTTTCTTCTG2910.11887594825015421No Hit
ATGCAAGCTGGTACCAGCAGAAACCAGGACAGGCCCCTTTACTTGTCGTC2880.1176504230104619No Hit
GCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAAGACTCACCATGGAGTTTCGGCTG2870.11724191459723113No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC2850.1164248977707696No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA2830.11560788094430806No Hit
GCACTGAACACAGAAGACTCACCATGGAGTTTCGGCTGAGCTGGGTTTTCCTTGTAGCTATTTTAAAAGGTGCCC2820.11519937253107729No Hit
TTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT2820.11519937253107729No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG2750.11233981363846189No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC2750.11233981363846189No Hit
GATCAGCACTGAACACAGAAGACTCACCATGGAGTTTCGGCTGAGCTGGG2710.1107057799855388No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACGGTCACCACTGCTGTCCCGGGAGTTACAGTAAT2710.1107057799855388No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC2690.10988876315907725No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGGGAC2690.10988876315907725No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC2630.10743771267969263No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA2630.10743771267969263No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT2610.10662069585323108No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC2590.10580367902676956No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACGGTCACCACTGCTGTCCCGGGAGTTACAGTAAT2570.10498666220030801No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT2570.10498666220030801No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCACATATCAAAAGCACCGAAAT2540.1037611369606157No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA2510.10253561172092339No Hit
GAACACAGAAGACTCACCATGGAGTTTCGGCTGAGCTGGGTTTTCCTTGT2500.10212710330769262No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG2500.10212710330769262No Hit
GTATGTAGTACCACCACTGTAAATAACTGAGACCCATTCCAGCCCCTTCC2500.10212710330769262No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG2490.10171859489446185No Hit
AGATGAGGCTGACTATTACTGTAACTCCCGGGACAGCAGTGGTGACCGTTGGGTGTTCGGCGGAGGGACCA2480.10131008648123108No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA2460.10049306965476953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)