FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004683886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244793
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC8880.3627554709489242No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGACCTTTCATGTGAAGATACAGAGTGTTCTTCGAATTGTCTCTGG7150.2920835154600009No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA7130.2912664986335394No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6900.2818708051292316No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5590.2283562029960007No Hit
CTCTTTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT5340.21814349266523142No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA4840.19771807200369293No Hit
CTCCTCACTCTTTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGG4790.19567552993753906No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGGGACAGCCTCAGAAGG4370.1785181765818467No Hit
AACCAGGACAGGCCCCTTTACTTGTCGTCTATTATACAAACAGCCGGCCC4240.1732075672098467No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC4010.16381187370553896No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC3860.1576842475070774No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3840.15686723068061587No Hit
GTGTGAGGTGCAGCTTATGGAGTCTGGAGGAGGCTTGATCCAGCCTGGGGGGTCCCTGAGACTTTCCTGCGCAGC3800.15523319702769278No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3750.15319065496153894No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3740.15278214654830818No Hit
GTGTATTACTGTGCGAGAACGCCCGCGGACCCTAATTGTAGTTATTCCAA3680.15033109606892353No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC3580.14624601193661582No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG3580.14624601193661582No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT3560.14542899511015428No Hit
CTATTACTGTAACTCCCGGGACAGCAGTGGTGACCGTTGGGTGTTCGGCG3540.14461197828369274No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC3520.1437949614572312No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGGTGGAGCC3480.14216092780430814No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC3480.14216092780430814No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG3480.14216092780430814No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGG3350.1368503184323081No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGACCTTTCATGTGAAGATA3290.1343992679529235No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC3260.13317374271323118No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC3250.13276523430000042No Hit
ACTACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCGAAGAACACTCTGTATCTT3250.13276523430000042No Hit
GACTATTACTGTAACTCCCGGGACAGCAGTGGTGACCGTTGGGTGTTCGG3220.1315397090603081No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC3210.1311312006470773No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGGTGGAGCCAG3210.1311312006470773No Hit
CTATTATACAAACAGCCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCT3190.13031418382061577No Hit
ATTATACAAACAGCCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCACCTCAGGAAACACAGCTTCCTTG3090.1262290996883081No Hit
GTCCTGGTTTCTGCTGGTACCAGCTTGCATAATGCCTTCTGAGGCTGTCCCCTTGGCATGTGATCCTGACTGT3040.12418655762215422No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA3040.12418655762215422No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC3020.12336954079569268No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG2980.12173550714276961No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACGGTCACCACTGCTGTCCCGGGAGTTACAGTAAT2930.11969296507661575No Hit
ATGCAAGCTGGTACCAGCAGAAACCAGGACAGGCCCCTTTACTTGTCGTC2920.11928445666338498No Hit
GTATAATAGACGACAAGTAAAGGGGCCTGTCCTGGTTTCTGCTGGTACCAGCTTGCATAATGCCTTCTGAGG2900.11846743983692344No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG2890.11805893142369267No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC2890.11805893142369267No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG2850.1164248977707696No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT2840.11601638935753883No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGCTCTGTGGTTTCTTCTG2800.11438235570461575No Hit
GTATTACTGTGCGAGAACGCCCGCGGACCCTAATTGTAGTTATTCCAACT2800.11438235570461575No Hit
CTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGC2790.11397384729138497No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT2760.11274832205169266No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC2720.11111428839876956No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGGGACAGCCTCAGAAGGCATTATGCAAGCTGGT2710.1107057799855388No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG2700.11029727157230802No Hit
GATCAGCACTGAACACAGAAGACTCACCATGGAGTTTCGGCTGAGCTGGG2680.10948025474584648No Hit
GTAAAGGGGCCTGTCCTGGTTTCTGCTGGTACCAGCTTGCATAATGCCTT2680.10948025474584648No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGCTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG2610.10662069585323108No Hit
CTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGGGAC2600.10621218744000033No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC2600.10621218744000033No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG2570.10498666220030801No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA2560.10457815378707724No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA2550.10416964537384647No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG2550.10416964537384647No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG2530.10335262854738493No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCACATATCAAAAGCACCGAAAT2530.10335262854738493No Hit
GCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAAGACTCACCATGGAGTTTCGGCTG2510.10253561172092339No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG2490.10171859489446185No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT2480.10131008648123108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGT708.871475E-568.3383370
ACAAGCG200.00750190359.79604770
TTCTAAG252.5204124E-455.3879251
AAGTAGT200.007656218551.22216437
GTGGTAT452.048062E-645.3445171
GGTATCA1600.044.636011
TATGTGG1901.4048055E-744.06024670
ATGTGAT3850.043.4880370
GTGGCCG1951.7229104E-742.93049270
ACAGGTA405.477495E-542.4496962
GTATCAA5100.042.010361
GAGCCAT2905.4569682E-1141.2386570
GATATGT2103.0827323E-739.8640370
TATCAAC5400.039.6197132
CGGGTGT350.001451376138.82701514
GACAGGT558.133155E-637.100061
TGGTATC558.213427E-637.0470052
TCTTGTT1958.514518E-636.79756570
ATCAACG5950.036.528143
TCAACGC5950.036.528144
Produced by FastQC (version 0.11.5)