FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004683889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004683889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927931
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGAC39760.4284801348376118No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAAT31250.3367707297202055No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30760.33149016467819264No Hit
GGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGC29880.3220067009292717No Hit
GTAATACACAGCCGTGTCTTCGGCCTTCAGACTGTTCATTTGTAGATACACTGAACTCTTGGCGTCGTCTCTGGA28800.3103679045101414No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28630.3085358717404635No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28220.3041174397665344No Hit
GGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26670.2874136115724122No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA23980.2584243871580969No Hit
GTGTATTACTGTGCGAGAGGTCGTCCTTTCACATTTTTCCGTGAGACAAG23380.25195838914746893No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC23330.2514195559799166No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCAC22850.24624675757141423No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC20140.21704199989007805No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19390.2089595023767931No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18960.2043255371358431No Hit
CCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGA18510.1994760386278721No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC18510.1994760386278721No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT18130.1953809065544744No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACT17930.1932255738842651No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17790.19171684101511857No Hit
GGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAT17740.19117800784756625No Hit
GAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG17700.19074694131352438No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG17310.18654404260661622No Hit
CCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCT17180.18514307637098015No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC17060.1838498767688546No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17000.1832032769677918No Hit
CCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC16960.18277221043374994No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG16570.17856931172684176No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG16040.1728576801507871No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16000.1724266136167452No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGGCCGTGTTCCC15890.1712411806481301No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15680.1689780813444103No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCC15610.16822371490983704No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATA15500.16703828194122192No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15410.16606838223962775No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15240.1642363494699498No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15200.16380528293590796No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTT14750.15895578442793698No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACA14640.15777035145932186No Hit
GTTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACC14520.1564771518571963No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAACAGGGTCGA14510.1563693852236858No Hit
CTTTTAGTTCCGATGTTGTTCCCCGCACAGATAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG14490.15615385195666487No Hit
GCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA14380.15496841898804975No Hit
CGGCCACCCTGACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTAT13970.15054998701412067No Hit
CCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATAATAGTCGGCCTCAT13970.15054998701412067No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13930.1501189204800788No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13700.14764028790933809No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13500.14548495523912877No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACCATCATCAT13410.14451505553753458No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13290.14322185593540898No Hit
GACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCAT13250.14279078940136714No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13220.14246748950083574No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGC13130.14149758979924154No Hit
GGCCAGGCCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC12530.1350315917886136No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA12510.13481605852159267No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC12480.13449275862106128No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12470.1343849919875508No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC12450.13416945872052988No Hit
CAGTAATACACAGCCGTGTCTTCGGCCTTCAGACTGTTCATTTGTAGATA12320.1327684924848938No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTA12320.1327684924848938No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG12210.1315830595162787No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11980.12910442694553798No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA11850.12770346070990193No Hit
TCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC11760.1267335610083077No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11740.1265180277412868No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGATCCACGTCAGCCAAAAAATTTCAAAGGGGAATCCAGAGGCTGCAC11700.12608696120724494No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11560.12457822833809842No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11540.12436269507107749No Hit
CTCCTGTGCAGCCTCTGGATTCCCCTTTGAAATTTTTTGGCTGACGTGGATCCGCCAGGCTCCAGGGAAGGGG11510.12403939517054607No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT11460.12350056200299375No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT11450.12339279536948329No Hit
GTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT11430.12317726210246235No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACC11350.12231512903437863No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAA11270.12145299596629491No Hit
GTATTACTGTGCGAGAGGTCGTCCTTTCACATTTTTCCGTGAGACAAGTGGTTATGCCAGTTTTGACTACTGGGG11080.11940542992959606No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11010.1186510634950228No Hit
GAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCC11000.11854329686151234No Hit
CAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC10930.11778893042693908No Hit
CTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCT10910.11757339715991813No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10890.11735786389289721No Hit
GTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAA10850.11692679735885533No Hit
GTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGG10590.11412486488758322No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10520.11337049845300998No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGT10450.11261613201843672No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10280.11078409924875879No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG10220.110137499447696No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10190.1098141995471646No Hit
CTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG10130.10916759974610181No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAA10130.10916759974610181No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9930.1070122670758925No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC9910.10679673380887156No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9860.10625790064131924No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG9730.1048569344056832No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTG9700.10453363450515177No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9590.10334820153653666No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG9570.10313266826951573No Hit
ACATAAGCCCAGATGGAAGTGAGAAATATTATGTAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGACGACG9550.1029171350024948No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9460.10194723530090061No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTC9460.10194723530090061No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9440.10173170203387968No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9300.10022296916473315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)